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pgl3 control firefly luciferase vector  (Vazyme Biotech Co)
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Pgl3 Control Firefly Luciferase Vector, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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firefly luciferase reporter vectors  (Genechem)
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Detachment‐induced NNMT is upregulated by the FAK‐STAT3 axis. (A) Representative image of the positive correlation between FAK and STAT3 in breast cancer and pan‐cancer according to the GEPIA 2 database analysis. (B) Representative results of STAT3, P‐STAT3, FAK, and P‐FAK proteins by WB in the detached BT‐549, MCF‐7, and MDA‐MB‐231 cells. (C,D) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m ) and P‐STAT3 inhibitor C188‐9 (10 µ m ) for 48 h by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (E,F) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m for 48 h) and P‐STAT3 activator (1 µ m for 24 h) by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (G,H) ChIP assay using P‐STAT3 antibodies in MDA‐MB‐231 cells cultured under suspension conditions was performed, followed by PCR and qPCR, which showed P‐STAT3 enrichment at the NNMT promoter vs. IgG control. ** p < 0.01. I: <t>Dual‐luciferase</t> reporter assays showed that co‐transfection with the full‐length NNMT promoter and a STAT3‐expressing plasmid significantly enhanced luciferase activity, whereas this enhancement was abolished when a truncated NNMT promoter was used. Data were presented as mean ± SEM. **** p < 0.0001.
Firefly Luciferase Reporter Vectors, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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firefly luciferase reporter plasmid vectors  (Genechem)
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Genechem firefly luciferase reporter plasmid vectors
Detachment‐induced NNMT is upregulated by the FAK‐STAT3 axis. (A) Representative image of the positive correlation between FAK and STAT3 in breast cancer and pan‐cancer according to the GEPIA 2 database analysis. (B) Representative results of STAT3, P‐STAT3, FAK, and P‐FAK proteins by WB in the detached BT‐549, MCF‐7, and MDA‐MB‐231 cells. (C,D) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m ) and P‐STAT3 inhibitor C188‐9 (10 µ m ) for 48 h by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (E,F) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m for 48 h) and P‐STAT3 activator (1 µ m for 24 h) by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (G,H) ChIP assay using P‐STAT3 antibodies in MDA‐MB‐231 cells cultured under suspension conditions was performed, followed by PCR and qPCR, which showed P‐STAT3 enrichment at the NNMT promoter vs. IgG control. ** p < 0.01. I: <t>Dual‐luciferase</t> reporter assays showed that co‐transfection with the full‐length NNMT promoter and a STAT3‐expressing plasmid significantly enhanced luciferase activity, whereas this enhancement was abolished when a truncated NNMT promoter was used. Data were presented as mean ± SEM. **** p < 0.0001.
Firefly Luciferase Reporter Plasmid Vectors, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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firefly luciferase reporter plasmid vectors - by Bioz Stars, 2026-06
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plasmid pgl3-basic vectors carrying the firefly luciferase reporter  (Promega)
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Detachment‐induced NNMT is upregulated by the FAK‐STAT3 axis. (A) Representative image of the positive correlation between FAK and STAT3 in breast cancer and pan‐cancer according to the GEPIA 2 database analysis. (B) Representative results of STAT3, P‐STAT3, FAK, and P‐FAK proteins by WB in the detached BT‐549, MCF‐7, and MDA‐MB‐231 cells. (C,D) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m ) and P‐STAT3 inhibitor C188‐9 (10 µ m ) for 48 h by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (E,F) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m for 48 h) and P‐STAT3 activator (1 µ m for 24 h) by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (G,H) ChIP assay using P‐STAT3 antibodies in MDA‐MB‐231 cells cultured under suspension conditions was performed, followed by PCR and qPCR, which showed P‐STAT3 enrichment at the NNMT promoter vs. IgG control. ** p < 0.01. I: <t>Dual‐luciferase</t> reporter assays showed that co‐transfection with the full‐length NNMT promoter and a STAT3‐expressing plasmid significantly enhanced luciferase activity, whereas this enhancement was abolished when a truncated NNMT promoter was used. Data were presented as mean ± SEM. **** p < 0.0001.
Plasmid Pgl3 Basic Vectors Carrying The Firefly Luciferase Reporter, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmir-report firefly luciferase vector  (Azenta)
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Detachment‐induced NNMT is upregulated by the FAK‐STAT3 axis. (A) Representative image of the positive correlation between FAK and STAT3 in breast cancer and pan‐cancer according to the GEPIA 2 database analysis. (B) Representative results of STAT3, P‐STAT3, FAK, and P‐FAK proteins by WB in the detached BT‐549, MCF‐7, and MDA‐MB‐231 cells. (C,D) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m ) and P‐STAT3 inhibitor C188‐9 (10 µ m ) for 48 h by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (E,F) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m for 48 h) and P‐STAT3 activator (1 µ m for 24 h) by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (G,H) ChIP assay using P‐STAT3 antibodies in MDA‐MB‐231 cells cultured under suspension conditions was performed, followed by PCR and qPCR, which showed P‐STAT3 enrichment at the NNMT promoter vs. IgG control. ** p < 0.01. I: <t>Dual‐luciferase</t> reporter assays showed that co‐transfection with the full‐length NNMT promoter and a STAT3‐expressing plasmid significantly enhanced luciferase activity, whereas this enhancement was abolished when a truncated NNMT promoter was used. Data were presented as mean ± SEM. **** p < 0.0001.
Pmir Report Firefly Luciferase Vector, supplied by Azenta, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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firefly luciferase reporter plasmids (plasmid backbone, plv‑cmv‑luciferase‑t2a‑hygro vector  (Shanghai GenePharma)
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Shanghai GenePharma firefly luciferase reporter plasmids (plasmid backbone, plv‑cmv‑luciferase‑t2a‑hygro vector
Detachment‐induced NNMT is upregulated by the FAK‐STAT3 axis. (A) Representative image of the positive correlation between FAK and STAT3 in breast cancer and pan‐cancer according to the GEPIA 2 database analysis. (B) Representative results of STAT3, P‐STAT3, FAK, and P‐FAK proteins by WB in the detached BT‐549, MCF‐7, and MDA‐MB‐231 cells. (C,D) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m ) and P‐STAT3 inhibitor C188‐9 (10 µ m ) for 48 h by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (E,F) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m for 48 h) and P‐STAT3 activator (1 µ m for 24 h) by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (G,H) ChIP assay using P‐STAT3 antibodies in MDA‐MB‐231 cells cultured under suspension conditions was performed, followed by PCR and qPCR, which showed P‐STAT3 enrichment at the NNMT promoter vs. IgG control. ** p < 0.01. I: <t>Dual‐luciferase</t> reporter assays showed that co‐transfection with the full‐length NNMT promoter and a STAT3‐expressing plasmid significantly enhanced luciferase activity, whereas this enhancement was abolished when a truncated NNMT promoter was used. Data were presented as mean ± SEM. **** p < 0.0001.
Firefly Luciferase Reporter Plasmids (Plasmid Backbone, Plv‑Cmv‑Luciferase‑T2a‑Hygro Vector, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reporter mixture containing serum response element (sre) firefly luciferase and renilla vectors  (Qiagen)
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Qiagen reporter mixture containing serum response element (sre) firefly luciferase and renilla vectors
Detachment‐induced NNMT is upregulated by the FAK‐STAT3 axis. (A) Representative image of the positive correlation between FAK and STAT3 in breast cancer and pan‐cancer according to the GEPIA 2 database analysis. (B) Representative results of STAT3, P‐STAT3, FAK, and P‐FAK proteins by WB in the detached BT‐549, MCF‐7, and MDA‐MB‐231 cells. (C,D) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m ) and P‐STAT3 inhibitor C188‐9 (10 µ m ) for 48 h by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (E,F) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m for 48 h) and P‐STAT3 activator (1 µ m for 24 h) by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (G,H) ChIP assay using P‐STAT3 antibodies in MDA‐MB‐231 cells cultured under suspension conditions was performed, followed by PCR and qPCR, which showed P‐STAT3 enrichment at the NNMT promoter vs. IgG control. ** p < 0.01. I: <t>Dual‐luciferase</t> reporter assays showed that co‐transfection with the full‐length NNMT promoter and a STAT3‐expressing plasmid significantly enhanced luciferase activity, whereas this enhancement was abolished when a truncated NNMT promoter was used. Data were presented as mean ± SEM. **** p < 0.0001.
Reporter Mixture Containing Serum Response Element (Sre) Firefly Luciferase And Renilla Vectors, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reporter vector carrying firefly luciferase  (Promega)
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Promega reporter vector carrying firefly luciferase
Detachment‐induced NNMT is upregulated by the FAK‐STAT3 axis. (A) Representative image of the positive correlation between FAK and STAT3 in breast cancer and pan‐cancer according to the GEPIA 2 database analysis. (B) Representative results of STAT3, P‐STAT3, FAK, and P‐FAK proteins by WB in the detached BT‐549, MCF‐7, and MDA‐MB‐231 cells. (C,D) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m ) and P‐STAT3 inhibitor C188‐9 (10 µ m ) for 48 h by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (E,F) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m for 48 h) and P‐STAT3 activator (1 µ m for 24 h) by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (G,H) ChIP assay using P‐STAT3 antibodies in MDA‐MB‐231 cells cultured under suspension conditions was performed, followed by PCR and qPCR, which showed P‐STAT3 enrichment at the NNMT promoter vs. IgG control. ** p < 0.01. I: <t>Dual‐luciferase</t> reporter assays showed that co‐transfection with the full‐length NNMT promoter and a STAT3‐expressing plasmid significantly enhanced luciferase activity, whereas this enhancement was abolished when a truncated NNMT promoter was used. Data were presented as mean ± SEM. **** p < 0.0001.
Reporter Vector Carrying Firefly Luciferase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Detachment‐induced NNMT is upregulated by the FAK‐STAT3 axis. (A) Representative image of the positive correlation between FAK and STAT3 in breast cancer and pan‐cancer according to the GEPIA 2 database analysis. (B) Representative results of STAT3, P‐STAT3, FAK, and P‐FAK proteins by WB in the detached BT‐549, MCF‐7, and MDA‐MB‐231 cells. (C,D) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m ) and P‐STAT3 inhibitor C188‐9 (10 µ m ) for 48 h by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (E,F) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m for 48 h) and P‐STAT3 activator (1 µ m for 24 h) by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (G,H) ChIP assay using P‐STAT3 antibodies in MDA‐MB‐231 cells cultured under suspension conditions was performed, followed by PCR and qPCR, which showed P‐STAT3 enrichment at the NNMT promoter vs. IgG control. ** p < 0.01. I: Dual‐luciferase reporter assays showed that co‐transfection with the full‐length NNMT promoter and a STAT3‐expressing plasmid significantly enhanced luciferase activity, whereas this enhancement was abolished when a truncated NNMT promoter was used. Data were presented as mean ± SEM. **** p < 0.0001.

Journal: Advanced Science

Article Title: Detachment‐Induced FAK‐STAT3‐NNMT Inhibits CTCs Anoikis to Promote Breast Cancer Metastasis by Enhancing Fatty Acid Oxidation

doi: 10.1002/advs.202522837

Figure Lengend Snippet: Detachment‐induced NNMT is upregulated by the FAK‐STAT3 axis. (A) Representative image of the positive correlation between FAK and STAT3 in breast cancer and pan‐cancer according to the GEPIA 2 database analysis. (B) Representative results of STAT3, P‐STAT3, FAK, and P‐FAK proteins by WB in the detached BT‐549, MCF‐7, and MDA‐MB‐231 cells. (C,D) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m ) and P‐STAT3 inhibitor C188‐9 (10 µ m ) for 48 h by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (E,F) The protein and mRNA levels of NNMT and P‐STAT3 after treating detached cells with FAK inhibitor PF‐562271 (10 µ m for 48 h) and P‐STAT3 activator (1 µ m for 24 h) by WB and QPCR. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (G,H) ChIP assay using P‐STAT3 antibodies in MDA‐MB‐231 cells cultured under suspension conditions was performed, followed by PCR and qPCR, which showed P‐STAT3 enrichment at the NNMT promoter vs. IgG control. ** p < 0.01. I: Dual‐luciferase reporter assays showed that co‐transfection with the full‐length NNMT promoter and a STAT3‐expressing plasmid significantly enhanced luciferase activity, whereas this enhancement was abolished when a truncated NNMT promoter was used. Data were presented as mean ± SEM. **** p < 0.0001.

Article Snippet: All plasmids used in this assay, including the firefly luciferase reporter vectors (e.g., pGL4‐basic containing the wild‐type or truncated NNMT promoter), the STAT3 expression plasmid, and the Renilla luciferase internal control plasmid (pRL‐TK), were custom‐synthesized by GeneChem Co., Ltd. (Shanghai, China).

Techniques: Cell Culture, Suspension, Control, Luciferase, Cotransfection, Expressing, Plasmid Preparation, Activity Assay